Abstract
The structure of isoselagine, isolated from the Chinese medicinal plant Lycopodium serratum Thunb. var. longipetiolatum Spring, was shown to be identical with huperzine A (1) and to have an exocyclic double bond in the E -configuration from NOE data. In the process of extraction of isoselagine, we also
isolated and identified another alkaloid, 6α-hydroxylycopodine (L. 20) (2) that was found to occur in this plant for the first time. The structure of L. 20
was previously determined mainly by the chemical means, and this is the first report
on the analysis of its structure by 1H- and 13C-NMR with more advanced 2D-NMR techniques. The X-ray crystallography revealed the
stereochemistry of 2 and confirmed its structure. The hemostatic properties of the isolated alkaloids
were studied, but they showed no platelet aggregation activity.
Key words
Lycopodium serratum Thunb var. longipetiolatum Spring - isoselagine - huperzine A - 6α-hy-droxylycopodine - stereochemistry - NMR-assignments
- X-ray analysis
